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Nasal fibroblasts express costimulatory factors. Surgically removed nasal mucosal tissues were collected, from which fibroblasts were isolated. A, gated FCM plots are CD80 + , or CD83 + , or <t>CD86</t> + fibroblasts. B, bars show mean ± SD of CD80 + , or CD83 + , or CD86 + fibroblasts of 10 samples per group. Each dot in bars presents one sample. Statistics: ANOVA + Dunnett's test. p values are presented in figures. C, protein samples were extracted from purified fibroblasts, and analyzed by Western blotting. Immunoblots show CD80, CD83, and CD86 proteins in fibroblast's extracts. The data of immunoblots are from one experiment that represent three independent experiments with pooled protein from 10 samples per group. Abbreviations: NC: Normal control. AR: Allergic rhinitis. nAR: Non-AR patients. AR: AR patients. FCM: Flow cytometry.
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Nasal fibroblasts express costimulatory factors. Surgically removed nasal mucosal tissues were collected, from which fibroblasts were isolated. A, gated FCM plots are CD80 + , or CD83 + , or CD86 + fibroblasts. B, bars show mean ± SD of CD80 + , or CD83 + , or CD86 + fibroblasts of 10 samples per group. Each dot in bars presents one sample. Statistics: ANOVA + Dunnett's test. p values are presented in figures. C, protein samples were extracted from purified fibroblasts, and analyzed by Western blotting. Immunoblots show CD80, CD83, and CD86 proteins in fibroblast's extracts. The data of immunoblots are from one experiment that represent three independent experiments with pooled protein from 10 samples per group. Abbreviations: NC: Normal control. AR: Allergic rhinitis. nAR: Non-AR patients. AR: AR patients. FCM: Flow cytometry.

Journal: Innate Immunity

Article Title: Nasal mucosal fibroblasts produce IL-4 to induce Th2 response

doi: 10.1177/17534259241254623

Figure Lengend Snippet: Nasal fibroblasts express costimulatory factors. Surgically removed nasal mucosal tissues were collected, from which fibroblasts were isolated. A, gated FCM plots are CD80 + , or CD83 + , or CD86 + fibroblasts. B, bars show mean ± SD of CD80 + , or CD83 + , or CD86 + fibroblasts of 10 samples per group. Each dot in bars presents one sample. Statistics: ANOVA + Dunnett's test. p values are presented in figures. C, protein samples were extracted from purified fibroblasts, and analyzed by Western blotting. Immunoblots show CD80, CD83, and CD86 proteins in fibroblast's extracts. The data of immunoblots are from one experiment that represent three independent experiments with pooled protein from 10 samples per group. Abbreviations: NC: Normal control. AR: Allergic rhinitis. nAR: Non-AR patients. AR: AR patients. FCM: Flow cytometry.

Article Snippet: shRNA kits of IL4RA and CD28, antibodies (Abs) of EpCAM [Cat#: sc-25308, fluorochrome: Alexa Fluor (AF) 488], CD45 (sc-1178, AF546), PDPN (sc-53533, AF594), CD3 (sc-20047, AF648), CD19 (sc-390244, AF680), CD11c (sc-46676, AF700), CD80 (sc-376012, AF594), CD83 (sc-55536, AF648), CD86 (sc-28347, AF700), IL-4 (sc-12723, AF488), CD4 (sc-19641, AF546), IL4RA (sc-28361) and CD28 (sc-70612) were purchased from Santa Cruz Biotech (Santa Cruz, CA).

Techniques: Isolation, Purification, Western Blot, Control, Flow Cytometry